Adult Neurogenesis
Produces Neurons with Unique GABAergic Synapses in the Olfactory Bulb.
Valley MT, Henderson
LG, Inverso SA, Lledo PM.J Neurosci. 2013 Sep 11;33(37):14660-5. PMID: 24027267
Objective: In adult mammals, interneurons are continuously generated in the olfactory bulb, many of which later become granule cells. It is known that the immature cells are integrated in to neural circuits and receive inputs that contribute to whether or not they will live or die, but little has been shown about the differences in this fate-deciding process between early and late stages of an organism’s life. In this paper, it was found that regulation by the GABAB receptor mediated presynaptic regulation is absent in adult-born cells, but not in cells born during neurogenesis.
Results:
·
Recordings from postsynaptic mitral cells (MCs)
during photostimulation of channelrhodopsin-expressing presynaptic granule
cells (GCs) showed inhibitory post synaptic currents (IPSCs) that could be
blocked by GABAA antagonists or application of TTX, suggesting that
photostimulation caused GABA release from GCs on to MCs.
·
Application of the GABAB receptor agonist
R/S-baclofen caused a decrease in IPSCS caused by photostimulation of
early-born GCs, but not in GCs born at postnatal day 60. They found that GABAB receptor function was
not linked to the age of the cell, but rather that aged early-born cells
retained receptor function while younger late-born cells did not have GABAB
receptor function.
·
After lentiviral expression of markers to
differentiate GCs born on p6 and p60, immunoreactivity for the GABAB1 subunit
of GABAB was assayed. P60 GCs had
greater dendritic and internal GABAB1, while p6 GCs had greater axonal GABAB1. This shows that GCs born at different times throughout
an animal’s life can have different distribution of the same receptor.
Methods: Mice were anesthetized and their olfactory
bulbs were given bilateral injections of lentivirus containing a cassette that
had channelrhdopsin2(H134R)eYFP under the control of the synapsin
promoter. After allowing time for gene
expression, slices were made from the olfactory bulbs and MCs were held under
voltage clamp at +10mV in order to monitor IPSCs. In animals used for immunohistochemistry, the
brain was removed and fixed with 4% paraformaldehyde.
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