Thursday, June 20, 2019

Acute sympathoexcitatory action of angiotensin II in conscious baroreceptor-denervated rats


By Ling Xu and Alan F. Sved

Angiotensin II (ANG II) levels have two competing influences on sympathetic outflow. ANG II seems to increase SNA. However, it also acts as a vasoconstrictor to increases MAP, which in turn stimulates baroreceptors, thereby inhibiting SNA. This makes it hard to determine the direct actions of ANG II. In order to determine what it directly effects, this experiment administered ANG II to sinoaortic-denervated rats (no baroreflex). 

Baseline MAP was higher in SAD than control rats. In control rats, infusion of ANG II rapidly increased MAP, which was accompanied by bradychardia and sympathoinhibition. Over time, HR and LSNA slowly returned to normal by the end of the infusion period. In contrast, denervated rats had a larger initial increase in MAP upon ANG II injection. However, the increase in MAP was accompanied by increased HR and LSNA, rather than the decrease seen in the control group. This indicates that ANG II can produce rapid sympathoexcitation. There are several sites at which ANG II could act to increase SNA and HR. It has been shown to act directly on the heart in high concentrations. Alternatively, in might increase HR by increasing sympathetic neural activity to the heart and/or decreasing parasympathetic neural activity to the heart. ANG II may act on areas of the CNS lacking a blood brain barrier, such as the area postrema. I will expand on its effects on the CNS in future blog posts.

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