In this paper, they wanted to figure out what they believed to be the best way to do MeMRI - using intracerebroventricular administration (ICV) of Mn, similar to what we have recently discussed.
They conducted their by using T1-Weighted scans following 50uL injections of 20, 30, 40, 60, and 80mM MnCl2 in to the cisterna magna, tracing signal enhancement over the next 4 days. They found that 20mM injections produced the fewest toxic side effects and that larger concentrations were sometimes lethal. Signal enhancement was seen as early as 1hr after Mn administration, but only in circumventricular areas. After 7hr there was a general, but heterogenous, signal enhancement. The signal continued to spread out, but was too intense to analyze at 14 and 19hrs. Their best enhancement was at 24hr after injection, and the signal began to decrease until they stopped imaging at 96hr post-administration.
So this paper was cool because they answered a couple of questions I've been having. However, it did not address activity-dependent uptake like I would want to do. It seems like their entire goal was to look at brain structure, regardless of the effect on the animal. For the functional studies I would like to do, it might be better to use a lower dose of Mn and measure at 12-14hours. -DH
Saturday, May 17, 2014
New method of manganese-enhanced Magnetic Resonance Imaging (MEMRI) for rat brain research.
Exp Anim. 2012;61(2):157-64.
Jeong KY, Lee C, Cho JH, Kang JH, Na HS.
In this paper, they wanted to figure out what they believed to be the best way to do MeMRI - using intracerebroventricular administration (ICV) of Mn, similar to what we have recently discussed.
They conducted their by using T1-Weighted scans following 50uL injections of 20, 30, 40, 60, and 80mM MnCl2 in to the cisterna magna, tracing signal enhancement over the next 4 days. They found that 20mM injections produced the fewest toxic side effects and that larger concentrations were sometimes lethal. Signal enhancement was seen as early as 1hr after Mn administration, but only in circumventricular areas. After 7hr there was a general, but heterogenous, signal enhancement. The signal continued to spread out, but was too intense to analyze at 14 and 19hrs. Their best enhancement was at 24hr after injection, and the signal began to decrease until they stopped imaging at 96hr post-administration.
So this paper was cool because they answered a couple of questions I've been having. However, it did not address activity-dependent uptake like I would want to do. It seems like their entire goal was to look at brain structure, regardless of the effect on the animal. For the functional studies I would like to do, it might be better to use a lower dose of Mn and measure at 12-14hours. -DH
In this paper, they wanted to figure out what they believed to be the best way to do MeMRI - using intracerebroventricular administration (ICV) of Mn, similar to what we have recently discussed.
They conducted their by using T1-Weighted scans following 50uL injections of 20, 30, 40, 60, and 80mM MnCl2 in to the cisterna magna, tracing signal enhancement over the next 4 days. They found that 20mM injections produced the fewest toxic side effects and that larger concentrations were sometimes lethal. Signal enhancement was seen as early as 1hr after Mn administration, but only in circumventricular areas. After 7hr there was a general, but heterogenous, signal enhancement. The signal continued to spread out, but was too intense to analyze at 14 and 19hrs. Their best enhancement was at 24hr after injection, and the signal began to decrease until they stopped imaging at 96hr post-administration.
So this paper was cool because they answered a couple of questions I've been having. However, it did not address activity-dependent uptake like I would want to do. It seems like their entire goal was to look at brain structure, regardless of the effect on the animal. For the functional studies I would like to do, it might be better to use a lower dose of Mn and measure at 12-14hours. -DH
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