J Neurosci Methods. 2003 Oct 15;129(1):73-9.
Central nervous system consists of a mixed population of
cells such as neurons, astrocytes and microglia. Immunolabelling was the only
way to characterize them until the authors from this study employed flow
cytometry to ascertain the proportion of different types of cells in primary
cultures. Specific antibodies were used against intracellular markers such as
Tuj-1 and GFAP to differentiate between neurons and astrocytes using flow
cytometry. An important advantage of flow cytometry is that it can be used for
quantitative studies of heterogeneous cultures. This technique will be
particularly useful for our lab since we can retrogradely label the spinally projecting C1
neurons using a tracer such as CtB and collect these cells specifically using
flow cytometry and perform future studies. Also it will be interesting to know
how different types of cells are populated within the rostrocaudal extent of
the RVLM.
-Madhan
No comments:
Post a Comment